4G0A
Crystallographic Analysis of Rotavirus NSP2-RNA Complex Reveals Specific Recognition of 5'-GG Sequence for RTPase activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-02-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 107.690, 107.690, 272.211 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.518 - 2.099 |
| R-factor | 0.1753 |
| Rwork | 0.174 |
| R-free | 0.20530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.654 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.520 | 2.140 |
| High resolution limit [Å] | 2.099 | 2.099 |
| Rmerge | 0.605 | |
| Number of reflections | 107465 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 50 mM sodium cacodylate, pH 6.5, 70 mM NaCl, 1 mM EDTA, 10-12% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
