4FLC
Structural and Biochemical Characterization of Human Adenylosuccinate Lyase (ADSL) and the R303C ADSL Deficiency Associated Mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.799, 105.562, 217.221 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.470 - 2.600 |
| R-factor | 0.2339 |
| Rwork | 0.230 |
| R-free | 0.29840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j91 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.009 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.370 | |
| Number of reflections | 58820 | |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 95.4 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris pH 8.5, 20% PEG 8000, 12.5 mM spermine tetrahydrochloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
