4F0A
Crystal structure of XWnt8 in complex with the cysteine-rich domain of Frizzled 8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-01-08 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.03325 |
Spacegroup name | P 41 |
Unit cell lengths | 110.070, 110.070, 82.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.640 - 3.250 |
R-factor | 0.2051 |
Rwork | 0.203 |
R-free | 0.23450 |
Structure solution method | MIRAS |
RMSD bond length | 0.005 |
RMSD bond angle | 0.880 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.640 | 3.300 |
High resolution limit [Å] | 3.250 | 3.250 |
Number of reflections | 29898 | |
<I/σ(I)> | 12.4 | 2 |
Completeness [%] | 97.7 | 98.7 |
Redundancy | 2.3 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 4-10 % (w/v) PEG 400 15-25 mM Zinc acetate 100 mM Sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |