4EMT

Crystal Structure of human STING bound to c-di-GMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	120
Detector technology	PIXEL
Collection date	2012-01-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9839
Spacegroup name	C 2 2 21
Unit cell lengths	69.797, 78.215, 128.222
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.250 - 1.500
R-factor	0.188
Rwork	0.188
R-free	0.21000
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.081
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.7_650)
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	54857
Completeness [%]	97.2	78.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	28% PEG400, 0.2M CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K