4EIL
Crystal Structure of the loop truncated Toxoplasma gondii TS-DHFR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9594 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.787, 144.399, 177.605 |
| Unit cell angles | 90.01, 89.93, 90.38 |
Refinement procedure
| Resolution | 48.403 - 2.197 |
| R-factor | 0.1855 |
| Rwork | 0.183 |
| R-free | 0.23330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eck |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.808 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.197 | 5.970 | 2.197 |
| Rmerge | 0.184 | 0.250 | 0.691 |
| Number of reflections | 265057 | ||
| <I/σ(I)> | 6 | ||
| Completeness [%] | 98.0 | 99.6 | 96.3 |
| Redundancy | 6.7 | 7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 290 | 10mg/ml protein, PEG 3350, Potassium formate, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
