4EDF
Dimeric hUGDH, K94E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2010-11-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.660, 151.070, 88.740 |
| Unit cell angles | 90.00, 107.01, 90.00 |
Refinement procedure
| Resolution | 43.306 - 2.080 |
| R-factor | 0.1868 |
| Rwork | 0.187 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q3e |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.735 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: dev_987)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 131092 | |
| <I/σ(I)> | 11.02 | 2.58 |
| Completeness [%] | 97.8 | 95.6 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 5 mM UDP-glucose, 200 mM magnesium chloride, 100 mM citrate tribasic dihydrate, pH 5.0, 10% PEG20000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
