4E4D
Crystal structure of mouse RANKL-OPG complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-01 |
Detector | NOIR-1 |
Wavelength(s) | 0.9951 |
Spacegroup name | P 63 |
Unit cell lengths | 109.763, 109.763, 78.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.700 - 2.700 |
R-factor | 0.202 |
Rwork | 0.194 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jtz |
RMSD bond length | 0.002 |
RMSD bond angle | 0.593 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
Rmerge | 0.084 | 0.029 | 0.563 |
Number of reflections | 14915 | ||
<I/σ(I)> | 12.8 | ||
Completeness [%] | 99.3 | 95.6 | 100 |
Redundancy | 2.9 | 2.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 293 | 100 mM sodium phosphate/citrate buffer, 14% polyethylene glycol 8000 and 250 mM sodium chloride, pH 4.10, VAPOR DIFFUSION, HANGING DROP, temperature 293K |