4E2J
X-Ray Crystal Structure of the Ancestral Glucocorticoid Receptor 2 ligand binding domain in complex with mometasone furoate and TIF-2 coactivator fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-11-12 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1 |
Spacegroup name | P 61 |
Unit cell lengths | 104.356, 104.356, 143.925 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.190 - 2.500 |
R-factor | 0.20706 |
Rwork | 0.205 |
R-free | 0.25483 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gn8 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.078 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 30710 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 1.5-3 M ammonium formate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |