4DVR
Crystal structure of YU2 gp120 core in complex with Fab 48d and NBD-557
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-12 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.445, 109.738, 130.682 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.400 - 2.500 |
| R-factor | 0.2333 |
| Rwork | 0.228 |
| R-free | 0.28870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rzk |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.359 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.134 | 0.497 |
| Number of reflections | 24559 | |
| <I/σ(I)> | 11.04 | 2.2 |
| Completeness [%] | 89.7 | 62.9 |
| Redundancy | 4.6 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG3350, 5% iso-propanol, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
