1RZK

HIV-1 YU2 GP120 ENVELOPE GLYCOPROTEIN COMPLEXED WITH CD4 AND INDUCED NEUTRALIZING ANTIBODY 17B

Summary for 1RZK

• Entry DOI: 10.2210/pdb1rzk/pdb
• Related: 1G9N 1RZ7 1RZ8 1RZF 1RZG 1RZI 1RZJ
• Descriptor: ENVELOPE GLYCOPROTEIN GP120, T-CELL SURFACE GLYCOPROTEIN CD4, ANTIBODY 17B, LIGHT CHAIN, ... (6 entities in total)
• Functional Keywords: complex (hiv envelope protein-cd4-fab); hiv-1 exterior envelope gp120 from laboratory-adapted isolate; hxbc2; surface t-cell glycoprotein cd4; antigen-binding fragment of human immunoglobulin 17b, viral protein-immune system complex, viral protein/immune system
• Biological source: Human immunodeficiency virus 1; More
• Total number of polymer chains: 4
• Total formula weight: 106296.15

Authors

Huang, C.C.,Venturi, M.,Majeed, S.,Moore, M.J.,Phogat, S.,Zhang, M.-Y.,Dimitrov, D.S.,Hendrickson, W.A.,Robinson, J.,Sodroski, J.,Wyatt, R.,Choe, H.,Farzan, M.,Kwong, P.D. (deposition date: 2003-12-24, release date: 2004-02-03, Last modification date: 2024-10-09)

Primary citation

Huang, C.C.,Venturi, M.,Majeed, S.,Moore, M.J.,Phogat, S.,Zhang, M.-Y.,Dimitrov, D.S.,Hendrickson, W.A.,Robinson, J.,Sodroski, J.,Wyatt, R.,Choe, H.,Farzan, M.,Kwong, P.D.
Structural basis of tyrosine sulfation and VH-gene usage in antibodies that recognize the HIV type 1 coreceptor-binding site on gp120
Proc.Natl.Acad.Sci.USA, 101:2706-2711, 2004

PubMed Abstract: The conserved surface of the HIV-1 gp120 envelope glycoprotein that binds to the HIV-1 coreceptor is protected from humoral recognition by multiple layers of camouflage. Here we present sequence and genomic analyses for 12 antibodies that pierce these defenses and determine the crystal structures of 5. The data reveal mechanisms and atomic-level details for three unusual immune features: posttranslational mimicry of coreceptor by tyrosine sulfation of antibody, an alternative molecular mechanism controlling such sulfation, and highly selective V(H)-gene usage. When confronted by extraordinary viral defenses, the immune system unveils novel adaptive capabilities, with tyrosine sulfation enhancing the vocabulary of antigen recognition.

PubMed: 14981267
DOI: 10.1073/pnas.0308527100

Experimental method

X-RAY DIFFRACTION (2.9 Å)