1RZK
HIV-1 YU2 GP120 ENVELOPE GLYCOPROTEIN COMPLEXED WITH CD4 AND INDUCED NEUTRALIZING ANTIBODY 17B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9721 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.980, 81.710, 74.480 |
| Unit cell angles | 90.00, 90.37, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 25.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.093 | 0.439 |
| Number of reflections | 23464 | |
| <I/σ(I)> | 10.38 | 2.33 |
| Completeness [%] | 98.5 | 97.1 |
| Redundancy | 4.37 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 293 | 0.5 UL OF PROTEIN (5 MG/ML IN 0.35 M NACL, 0.005 M TRIS CL PH 7.0) + 0.35 UL OF RESERVOIR (50 UL OF NA ACETATE PH 4.5 + 250 UL OF HAMPTON CRYSTAL SCREEN REAGENT 18 + 126 UL OF ETHANOL + 292 UL OF WATER), pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 2.5 (mM) | |
| 2 | 1 | drop | 0.35 (M) | ||
| 3 | 1 | drop | 0.02 (%(v/v)) | pH7.0 | |
| 4 | 1 | drop | protein | 4-8 (mg/ml) |
