4DF8
Crystal structure of the large fragment of DNA Polymerase I from Thermus aquaticus in a closed ternary complex with aminopentinyl-7-deaza-2-dATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.23000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 108.609, 108.609, 90.516 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.029 - 2.000 |
R-factor | 0.1966 |
Rwork | 0.195 |
R-free | 0.23390 |
Structure solution method | Difference Fourier Techniques |
Starting model (for MR) | 3m8s |
RMSD bond length | 0.014 |
RMSD bond angle | 0.993 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.7.3_928) |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.030 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
<I/σ(I)> | 14.43 | 1.78 |
Completeness [%] | 99.9 | 99.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 18% PEG 8000, 200mM Mg-Formate, 100 mMTris pH 8.0 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |