4D0Z

GalNAc-T2 crystal soaked with UDP-5SGalNAc, mEA2 and manganese (Higher resolution dataset)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	77
Spacegroup name	P 21 21 21
Unit cell lengths	116.485, 121.143, 249.387
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	249.390 - 2.200
R-factor	0.19743
Rwork	0.196
R-free	0.23377
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ffv
RMSD bond length	0.014
RMSD bond angle	1.884
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.090	0.820
Number of reflections	178474
<I/σ(I)>	14.5	2.6
Completeness [%]	99.8	100
Redundancy	8.6	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1