4D0F
Human Notch1 EGF domains 11-13 mutant T466A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-27 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 62.620, 62.620, 126.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.190 - 2.800 |
| R-factor | 0.2247 |
| Rwork | 0.223 |
| R-free | 0.26450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vj3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.330 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.300 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.120 | 0.440 |
| Number of reflections | 3956 | |
| <I/σ(I)> | 7.6 | 2.3 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 5.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | pH 4.5 |
