4CKI
Crystal Structure of oncogenic RET tyrosine kinase M918T bound to adenosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-26 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.893, 70.569, 78.997 |
| Unit cell angles | 90.00, 101.15, 90.00 |
Refinement procedure
| Resolution | 39.559 - 2.116 |
| R-factor | 0.1577 |
| Rwork | 0.156 |
| R-free | 0.19050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ivt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.901 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.210 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.070 | 0.180 |
| Number of reflections | 22084 | |
| <I/σ(I)> | 7.4 | 2.6 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 2M NA FORMATE, 0.1M NA CITRATE, PH 5.5 |
