4CAD
Mechanism of farnesylated CAAX protein processing by the integral membrane protease Rce1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-17 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | P 1 |
Unit cell lengths | 113.063, 90.087, 99.300 |
Unit cell angles | 89.06, 102.29, 90.00 |
Refinement procedure
Resolution | 42.343 - 2.500 |
R-factor | 0.2282 |
Rwork | 0.226 |
R-free | 0.26690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vg9 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.886 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.340 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.100 | 0.770 |
Number of reflections | 129829 | |
<I/σ(I)> | 11.1 | 2.5 |
Completeness [%] | 98.3 | 95.9 |
Redundancy | 9.2 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 12.5 MM MOPS PH 7.0, 350 MM NACL, 30% PEG 400 |