4CAD
Mechanism of farnesylated CAAX protein processing by the integral membrane protease Rce1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-17 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 |
| Unit cell lengths | 113.063, 90.087, 99.300 |
| Unit cell angles | 89.06, 102.29, 90.00 |
Refinement procedure
| Resolution | 42.343 - 2.500 |
| R-factor | 0.2282 |
| Rwork | 0.226 |
| R-free | 0.26690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vg9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.886 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.340 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.770 |
| Number of reflections | 129829 | |
| <I/σ(I)> | 11.1 | 2.5 |
| Completeness [%] | 98.3 | 95.9 |
| Redundancy | 9.2 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 12.5 MM MOPS PH 7.0, 350 MM NACL, 30% PEG 400 |
