4C9Z
Crystal structure of Siah1 at 1.95 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 2011-04-12 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 75.140, 104.590, 133.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.950 |
R-factor | 0.19967 |
Rwork | 0.198 |
R-free | 0.23855 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a25 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.302 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.990 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.040 | 0.180 |
Number of reflections | 38340 | |
<I/σ(I)> | 19.3 | 4.5 |
Completeness [%] | 99.3 | 96 |
Redundancy | 3.7 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | CRYSTALS GREW FROM EQUAL VOLUMES OF PROTEIN SOLUTION (15 MG ML-1 IN 15 MM TRIS-HCL PH7.5, 30 MM NACL AND 10 MM DTT) AND RESERVOIR SOLUTION (100 MES PH6.5, 1.5 M MGSO4). |