4C8K
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in a partially closed complex with the artificial base pair d5SICS-dNaMTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 115.039, 115.039, 91.106 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.637 - 2.170 |
| R-factor | 0.1832 |
| Rwork | 0.181 |
| R-free | 0.22140 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3m8s |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 2.290 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.100 | |
| Number of reflections | 34375 | |
| <I/σ(I)> | 14.44 | 0.47 |
| Completeness [%] | 91.1 | 51.2 |
| Redundancy | 6.6 | 4.7 |
| CC(1/2) | 0.998 | 0.478 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 15% W/V PEG 8000, 100MM TRIS PH 7.0, 200MM MAGNESIUM FORMIAT |
