4BZB
Crystal structure of the tetrameric dGTP-bound SAMHD1 mutant catalytic core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.203, 146.746, 98.723 |
| Unit cell angles | 90.00, 114.42, 90.00 |
Refinement procedure
| Resolution | 48.730 - 1.830 |
| R-factor | 0.1896 |
| Rwork | 0.189 |
| R-free | 0.20612 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u1n |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.112 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.100 | |
| Number of reflections | 197125 | |
| <I/σ(I)> | 14.8 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 100 MM SPG BUFFER, PH 7.4, 25% PEG 1500 |
