4BUR
Crystal structure of the reduced human Apoptosis inducing factor complexed with NAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Collection date | 2011-11-23 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 120.766, 120.766, 343.359 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.880 |
R-factor | 0.18014 |
Rwork | 0.176 |
R-free | 0.22973 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m6i |
RMSD bond length | 0.009 |
RMSD bond angle | 1.479 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 343.360 | 3.040 |
High resolution limit [Å] | 2.870 | 2.880 |
Rmerge | 0.100 | 0.470 |
Number of reflections | 66707 | |
<I/σ(I)> | 12.6 | 3.4 |
Completeness [%] | 99.8 | |
Redundancy | 6.6 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 16-20% PEG 4K, 0.2 M LI2SO4 AND 0.1 M TRIS-HCL PH 8.5 |