4BUR

Crystal structure of the reduced human Apoptosis inducing factor complexed with NAD

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016491	molecular_function	oxidoreductase activity
A	0046983	molecular_function	protein dimerization activity
B	0016491	molecular_function	oxidoreductase activity
B	0046983	molecular_function	protein dimerization activity
C	0016491	molecular_function	oxidoreductase activity
C	0046983	molecular_function	protein dimerization activity
D	0016491	molecular_function	oxidoreductase activity
D	0046983	molecular_function	protein dimerization activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	17
Details	BINDING SITE FOR RESIDUE NAD A 700

Chain	Residue
A	LEU267
A	ALA397
A	VAL398
A	GLY399
A	GLU453
A	HIS454
A	TRP483
A	FAD1612
A	HOH2006
A	GLY308
A	GLY309
A	PHE310
A	LEU311
A	GLU314
A	PRO335
A	GLU336
A	LYS342

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NAD A 701

Chain	Residue
A	TRP196
A	TRP483
A	GLU493
A	PHE582
A	ASN583

---

site_id	AC3
Number of Residues	18
Details	BINDING SITE FOR RESIDUE NAD B 700

Chain	Residue
B	LEU267
B	GLY307
B	GLY308
B	GLY309
B	PHE310
B	LEU311
B	GLU314
B	PRO335
B	GLU336
B	LYS342
B	ALA397
B	VAL398
B	GLY399
B	GLU453
B	HIS454
B	TRP483
B	FAD1612
B	HOH2004

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NAD B 701

Chain	Residue
B	TRP196
B	TRP483
B	GLU493
B	PHE582
B	ASN583

---

site_id	AC5
Number of Residues	17
Details	BINDING SITE FOR RESIDUE NAD C 700

Chain	Residue
C	LEU267
C	GLY308
C	GLY309
C	PHE310
C	LEU311
C	GLU314
C	PRO335
C	GLU336
C	LYS342
C	ALA397
C	VAL398
C	GLY399
C	GLU453
C	HIS454
C	TRP483
C	FAD1612
C	HOH2003

---

site_id	AC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE NAD C 701

Chain	Residue
A	ALA275
C	TRP196
C	TRP483
C	GLU493
C	PHE582
C	ASN583
C	HOH2007

---

site_id	AC7
Number of Residues	16
Details	BINDING SITE FOR RESIDUE NAD D 700

Chain	Residue
D	LEU267
D	GLY307
D	GLY308
D	GLY309
D	PHE310
D	LEU311
D	GLU314
D	PRO335
D	GLU336
D	ALA397
D	VAL398
D	GLY399
D	GLU453
D	HIS454
D	TRP483
D	FAD1612

---

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE NAD D 701

Chain	Residue
D	TRP196
D	ASP456
D	MET481
D	TRP483
D	GLU493
D	PHE582
D	ASN583

---

site_id	AC9
Number of Residues	28
Details	BINDING SITE FOR RESIDUE FAD A 1612

Chain	Residue
A	ARG172
A	PRO173
A	SER176
A	LYS177
A	VAL233
A	ALA259
A	THR260
A	GLY261
A	ARG285
A	GLY437
A	ASP438
A	GLU453
A	HIS454
A	HIS455
A	ALA458
A	PHE482
A	TRP483
A	NAD700
A	HOH2001
A	HOH2002
A	HOH2004
A	GLY138
A	GLY139
A	GLY140
A	THR141
A	ALA142
A	VAL162
A	GLU164

---

site_id	BC1
Number of Residues	26
Details	BINDING SITE FOR RESIDUE FAD B 1612

Chain	Residue
B	GLY138
B	GLY139
B	GLY140
B	THR141
B	ALA142
B	VAL162
B	GLU164
B	ARG172
B	PRO173
B	SER176
B	LYS231
B	LYS232
B	VAL233
B	ALA259
B	THR260
B	GLY261
B	PHE284
B	ARG285
B	GLY437
B	ASP438
B	GLU453
B	HIS454
B	HIS455
B	ALA458
B	PHE482
B	NAD700

---

site_id	BC2
Number of Residues	25
Details	BINDING SITE FOR RESIDUE FAD D 1612

Chain	Residue
D	GLY138
D	GLY139
D	GLY140
D	THR141
D	ALA142
D	VAL162
D	GLU164
D	ARG172
D	PRO173
D	SER176
D	LYS177
D	LYS232
D	VAL233
D	ALA259
D	THR260
D	GLY261
D	ARG285
D	GLY437
D	ASP438
D	GLU453
D	HIS454
D	HIS455
D	ALA458
D	PHE482
D	NAD700

---

site_id	BC3
Number of Residues	29
Details	BINDING SITE FOR RESIDUE FAD C 1612

Chain	Residue
C	GLY138
C	GLY139
C	GLY140
C	THR141
C	ALA142
C	VAL162
C	GLU164
C	ARG172
C	PRO173
C	SER176
C	LYS177
C	LYS231
C	LYS232
C	VAL233
C	ALA259
C	THR260
C	GLY261
C	ARG285
C	GLY437
C	ASP438
C	GLU453
C	HIS454
C	HIS455
C	ALA458
C	PHE482
C	TRP483
C	NAD700
C	HOH2001
C	HOH2002

---

site_id	BC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 1613

Chain	Residue
B	ARG584
D	ASN583
D	ARG584

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1396
Details	Region: {"description":"FAD-dependent oxidoreductase","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	Motif: {"description":"Nuclear localization signal","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12198487","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23217327","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24914854","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27178839","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27818101","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4BUR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4BV6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5KVI","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12198487","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23217327","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24914854","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27178839","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27818101","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4BUR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4BV6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5KVH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5KVI","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12198487","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24914854","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27178839","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27818101","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4BUR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4BV6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5KVI","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	36
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24914854","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4BUR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12198487","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23217327","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24914854","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27178839","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27818101","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4BUR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4BV6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5KVH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q9Z0X1","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12198487","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24914854","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27178839","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27818101","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4BUR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4BV6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FS9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5KVH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5KVI","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24914854","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4BUR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4BV6","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	8
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"Q9Z0X1","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"22103349","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---