4BPM
Crystal structure of a human integral membrane enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-02 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | H 3 2 |
Unit cell lengths | 86.400, 86.400, 181.120 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.639 - 2.080 |
R-factor | 0.2009 |
Rwork | 0.200 |
R-free | 0.21820 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.003 |
RMSD bond angle | 0.763 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.640 | 2.130 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.090 | 0.820 |
Number of reflections | 15904 | |
<I/σ(I)> | 9.9 | 2.5 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 5.1 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.7 | 277 | 8 %(V/V) 2-METHYL-2,4, -PENTANEDIOL (MPD), 0.4 M POTASSIUM NITRATE, 0.1 M POTASSIUM CITRATE, 0.1 M N-(CARBAMOYLMETHYL)IMINODIACETIC ACID (ADA) SODIUM PH 6.7. CRYSTALLIZED USING THE IN MESO (LIPIDIC CUBIC PHASE, LCP) METHOD AT 4 DEGREES CELCIUS WITH THE 8.8 MONOACYLGLYCEROL (8.8 MAG) DOPED WITH 2 MOL% OF DIOLEOYL PHOSPHATIDYLCHOLINE (DOPC) AS THE HOSTING LIPID. |