4BKX
The structure of HDAC1 in complex with the dimeric ELM2-SANT domain of MTA1 from the NuRD complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Collection date | 2011-10-06 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 108.199, 108.199, 133.164 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 76.750 - 3.000 |
| R-factor | 0.21337 |
| Rwork | 0.211 |
| R-free | 0.26149 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a69 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.023 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.600 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.160 | 0.660 |
| Number of reflections | 16916 | |
| <I/σ(I)> | 6 | 3.1 |
| Completeness [%] | 96.7 | 97.6 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M NA HEPES PH7.5, 2M AMMONIUM SULPHATE, 5% PEG400 |
