4BKF

crystal structure of the human EphA4 ectodomain in complex with human ephrinB3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 43 3 2
Unit cell lengths	300.530, 300.530, 300.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	106.250 - 4.650
R-factor	0.3286
Rwork	0.327
R-free	0.35080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 4BK4 3d12
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.000	4.700
High resolution limit [Å]	4.650	4.650
Rmerge	0.260
Number of reflections	25557
<I/σ(I)>	7.7	0.5
Completeness [%]	99.9	99.9
Redundancy	10.8	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				PROTEIN SAMPLE WAS MIXED 1:1:1 (V/V/V) WITH (0.08 M MAGNESIUM ACETATE TETRAHYDRATE. 0.05 M CACODYLATE PH 6.5, 15% POLYETHYLENE GLYCOL 400) AND 0.1 M SPERMIDINE (HAMPTON).
1				PROTEIN SAMPLE WAS MIXED 1:1:1 (V/V/V) WITH (0.08 M MAGNESIUM ACETATE TETRAHYDRATE. 0.05 M CACODYLATE PH 6.5, 15% POLYETHYLENE GLYCOL 400) AND 0.1 M SPERMIDINE (HAMPTON).