4BHW
Structural basis for autoinhibition of the acetyltransferase activity of p300
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-09 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.051, 92.894, 123.309 |
| Unit cell angles | 90.00, 98.43, 90.00 |
Refinement procedure
| Resolution | 46.620 - 2.799 |
| R-factor | 0.2096 |
| Rwork | 0.208 |
| R-free | 0.24410 |
| Structure solution method | SAD |
| Starting model (for MR) | 3biy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.102 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.070 | 0.590 |
| Number of reflections | 80344 | |
| <I/σ(I)> | 9.4 | 1.5 |
| Completeness [%] | 97.4 | 97.1 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM HEPES, PH 7.5, 20% PEG3350, 0.2M NACL |
