4AZA
Improved eIF4E binding peptides by phage display guided design.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU PROTEUM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-01 |
Detector | RIGAKU PT 135 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.662, 38.168, 121.758 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 121.760 - 2.160 |
R-factor | 0.23526 |
Rwork | 0.233 |
R-free | 0.27859 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2w97 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.142 |
Data reduction software | PROTEUM2 |
Data scaling software | PROTEUM2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.880 | 2.260 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.200 | 0.750 |
Number of reflections | 30410 | |
<I/σ(I)> | 5.22 | 1.38 |
Completeness [%] | 99.3 | 94.5 |
Redundancy | 9.22 | 6.51 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 20% PEG 5000 MME, 100 MM BISTRIS PH 6.5. |