4AUO
Crystal structure of MMP-1(E200A) in complex with a triple-helical collagen peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 76.671, 102.241, 80.734 |
| Unit cell angles | 90.00, 103.75, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.2111 |
| Rwork | 0.211 |
| R-free | 0.27270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2clt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.130 | 0.400 |
| Number of reflections | 20982 | |
| <I/σ(I)> | 5 | 2.2 |
| Completeness [%] | 86.8 | 77.9 |
| Redundancy | 1.7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8 |
