4AQ7
Ternary complex of E. coli leucyl-tRNA synthetase, tRNA(leu) and leucyl-adenylate analogue in the aminoacylation conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-29 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 158.660, 69.200, 228.840 |
Unit cell angles | 90.00, 104.35, 90.00 |
Refinement procedure
Resolution | 39.690 - 2.500 |
R-factor | 0.19075 |
Rwork | 0.188 |
R-free | 0.25031 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h3n |
RMSD bond length | 0.014 |
RMSD bond angle | 1.770 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.080 | 0.520 |
Number of reflections | 75711 | |
<I/σ(I)> | 11.1 | 2.1 |
Completeness [%] | 90.3 | 76.6 |
Redundancy | 2.9 | 2.16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 33 MICROM LEURS, 50 MICROM TRNALEU AND 1 MM LEUCYL-ADENYLATE ANALOGUE MIXED EQUALLY WITH 0.1 M BIS-TRIS PH 5.5, 23-25% W/V PEG 3350, AND 200 MM AMMONIUM ACETATE |