4AQ7
Ternary complex of E. coli leucyl-tRNA synthetase, tRNA(leu) and leucyl-adenylate analogue in the aminoacylation conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-29 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.660, 69.200, 228.840 |
| Unit cell angles | 90.00, 104.35, 90.00 |
Refinement procedure
| Resolution | 39.690 - 2.500 |
| R-factor | 0.19075 |
| Rwork | 0.188 |
| R-free | 0.25031 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h3n |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.770 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.520 |
| Number of reflections | 75711 | |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 90.3 | 76.6 |
| Redundancy | 2.9 | 2.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 33 MICROM LEURS, 50 MICROM TRNALEU AND 1 MM LEUCYL-ADENYLATE ANALOGUE MIXED EQUALLY WITH 0.1 M BIS-TRIS PH 5.5, 23-25% W/V PEG 3350, AND 200 MM AMMONIUM ACETATE |
