4A69
Structure of HDAC3 bound to corepressor and inositol tetraphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-18 |
| Detector | PILATUS 2D HYBRID ARRAY |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 86.447, 118.634, 190.707 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.350 - 2.060 |
| R-factor | 0.18955 |
| Rwork | 0.187 |
| R-free | 0.23617 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ew8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.261 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.930 | 2.170 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.100 | 0.330 |
| Number of reflections | 49237 | |
| <I/σ(I)> | 7.9 | 2.7 |
| Completeness [%] | 85.9 | 68.9 |
| Redundancy | 2.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5 0.2 M NACL 10 % V/V PROPAN-2-OL |
