4A64
Crystal structure of the N-terminal domain of human Cul4B at 2.57A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-03 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.248, 157.091, 92.916 |
| Unit cell angles | 90.00, 95.71, 90.00 |
Refinement procedure
| Resolution | 19.740 - 2.570 |
| R-factor | 0.1866 |
| Rwork | 0.184 |
| R-free | 0.22989 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1U6G 1ldk 1ldj 2hye |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.970 | 2.710 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.060 | 0.730 |
| Number of reflections | 58043 | |
| <I/σ(I)> | 18.6 | 2 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 24% PEG4000, 100 MM LISO4, 100 MM TRIS (PH 8.2) |
