4A5Y
Intermediate state of human kinesin Eg5 in complex with Ispinesib
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-03-08 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.630, 91.930, 163.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.603 - 2.450 |
| R-factor | 0.2042 |
| Rwork | 0.201 |
| R-free | 0.26780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x88 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.675 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.080 | 0.380 |
| Number of reflections | 48178 | |
| <I/σ(I)> | 18.4 | 4.4 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 8.7 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 14 % POLYETHYLENE GLYCOL-3350, 0.18 M AMMONIUM PHOSPHATE, pH 6.8 |
