4A04
Structure of the DNA-bound T-box domain of human TBX1, a transcription factor associated with the DiGeorge syndrome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.420, 92.740, 101.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.320 - 2.580 |
| R-factor | 0.2076 |
| Rwork | 0.206 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xbr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.060 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.400 | 2.640 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.090 | 0.950 |
| Number of reflections | 21470 | |
| <I/σ(I)> | 11.9 | 2.3 |
| Completeness [%] | 98.8 | 99.8 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 5% (W/V) POLYETHYLENE GLYCOL 6000, 100 MM CITRATE PH 5 |
