4ZUX

SAGA DUB module Ubp8/Sgf11/Sus1/Sgf73 bound to ubiqitinated nucleosome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-14
Detector	PSI PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	178.804, 179.174, 353.819
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.498 - 3.820
R-factor	0.2369
Rwork	0.234
R-free	0.25590
RMSD bond length	0.005
RMSD bond angle	1.023
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.500
High resolution limit [Å]	3.693
Rmerge	0.105
Number of reflections	121413
<I/σ(I)>	7.12
Completeness [%]	99.3
Redundancy	7.12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50 mM Tris-acetate pH 7.4, 50 mM sodium acetate, 5 mM Mg-acetate, 5% sucrose and 5% 2-propanol