4Z31

Crystal structure of the RC3H2 ROQ domain in complex with stem-loop and double-stranded forms of RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97924
Spacegroup name	P 1 21 1
Unit cell lengths	60.299, 174.734, 61.290
Unit cell angles	90.00, 114.12, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.2454
Rwork	0.245
R-free	0.25920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qik
RMSD bond length	0.007
RMSD bond angle	1.094
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.083	0.039	0.830
Rmeas	0.090	0.043	0.913
Rpim	0.036	0.018	0.377
Total number of observations	236295
Number of reflections	39757
<I/σ(I)>	8.4
Completeness [%]	99.1	99.7	97.6
Redundancy	5.9	6.2	5.4
CC(1/2)		0.963	0.801

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	The protein was diluted to 6.7 mg/ml then mixed with IER3 RNA at a ratio 1:2 before crystallized. 17% PEG10000, 0.1 M Bis-Tris pH6.5, 5% Ethylene glycol