4YZN
Humanized Roco4 bound to Compound 19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99995 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.400, 42.400, 326.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.640 - 1.550 |
| R-factor | 0.18887 |
| Rwork | 0.187 |
| R-free | 0.21595 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f0f |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.613 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0093) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.640 | 1.600 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.557 | |
| Number of reflections | 45260 | |
| <I/σ(I)> | 8.51 | 2.2 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 100 mM 1,3-bis(tris(hydroxymethyl)methylamino)propane pH 8.5 200mM Na/K tartrate |
