4YNQ

TREX1-dsDNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-13
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	83.142, 84.457, 103.000
Unit cell angles	90.00, 102.15, 90.00

Refinement procedure

Resolution	19.850 - 2.800
R-factor	0.2198
Rwork	0.218
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oa8
RMSD bond length	0.010
RMSD bond angle	1.516
Data reduction software	d*TREK
Data scaling software	d*TREK (9.9.9.1L)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.850	19.850	2.900
High resolution limit [Å]	2.800	5.980	2.800
Rmerge	0.102	0.066	0.533
Total number of observations	129430	13300	12505
Number of reflections	25822
<I/σ(I)>	5.4	14.6	1.2
Completeness [%]	94.9	97.8	90.5
Redundancy	3	3.5	2.56

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	PEG 4000