4YL6

Crystal structure of truncated cerebral cavernous malformation 2 C-terminal adaptor domain in complex with an internal helix of mitogen-activated protein kinase kinase kinase 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-04-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	61.210, 61.210, 68.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.780 - 2.100
R-factor	0.2049
Rwork	0.203
R-free	0.23720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ykd
RMSD bond length	0.002
RMSD bond angle	0.636
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.780	2.210
High resolution limit [Å]	2.100	2.100
Rmerge		0.298
Number of reflections	8073
<I/σ(I)>	31.2	5.9
Completeness [%]	99.4	96.4
Redundancy	11	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	3.5 M Sodium Formate