4Y7R

Crystal structure of WDR5 in complex with MYC MbIIIb peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-29
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	80.762, 87.177, 44.914
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.589 - 1.898
R-factor	0.1601
Rwork	0.159
R-free	0.18790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xl2
RMSD bond length	0.005
RMSD bond angle	0.958
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1951))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.589	1.930
High resolution limit [Å]	1.898	1.900
Rmerge		0.670
Number of reflections	25709
<I/σ(I)>	24.8	4.3
Completeness [%]	99.9	100
Redundancy	9.7	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.1 M Tris 7.0, 25% PEG 8K