4XST
Structure of the endoglycosidase-H treated L1-CR domains of the human insulin receptor in complex with residues 697-719 of the human insulin receptor (A-isoform)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 158.718, 158.718, 85.912 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.360 - 3.000 |
| R-factor | 0.2191 |
| Rwork | 0.217 |
| R-free | 0.26220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hr7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 79.360 | 6.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 4.000 | 3.000 |
| Rmerge | 0.500 | 0.372 | 0.893 |
| Rmeas | 0.381 | 9.140 | |
| Number of reflections | 13240 | 3959 | 1198 |
| <I/σ(I)> | 7.8 | 11.09 | 0.6 |
| Completeness [%] | 99.9 | 100 | 100 |
| Redundancy | 21.2 | 21.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2.45 M (NH4)2SO4 + 10 % glycerol |
