4XGZ
Crystal structure of human paxillin LD2 motif in complex with Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-10 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 |
Unit cell lengths | 108.189, 111.004, 143.865 |
Unit cell angles | 94.65, 95.28, 114.53 |
Refinement procedure
Resolution | 142.000 - 2.500 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pgf |
RMSD bond length | 0.006 |
RMSD bond angle | 1.073 |
Data reduction software | XDS (3.3.20) |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 142.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.721 | |
Number of reflections | 207767 | |
<I/σ(I)> | 7.4 | 1.1 |
Completeness [%] | 94.3 | 94.2 |
Redundancy | 2.3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.2 M sodium nitrate |