4WNK
Crystal Structure of Bovine G Protein Coupled-Receptor Kinase 5 in Complex with CCG215022
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.1271 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.106, 70.104, 182.825 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.470 - 2.420 |
| R-factor | 0.19635 |
| Rwork | 0.194 |
| R-free | 0.24297 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pnk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.483 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.136 | 0.936 |
| Number of reflections | 24217 | |
| <I/σ(I)> | 42.3 | 4.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 37.7 | 34.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | HEPES, ethylene glycol, PEG 3350 |
