4TU7
Structure of U2AF65 D231V variant with BrU5 DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 164.432, 37.361, 100.192 |
Unit cell angles | 90.00, 125.60, 90.00 |
Refinement procedure
Resolution | 35.982 - 2.087 |
R-factor | 0.1789 |
Rwork | 0.174 |
R-free | 0.22300 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.208 |
Data scaling software | SCALA (3.3.20) |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.447 | 39.447 | 2.200 |
High resolution limit [Å] | 2.087 | 6.600 | 2.087 |
Rmerge | 0.017 | 0.228 | |
Rmeas | 0.044 | ||
Rpim | 0.025 | 0.011 | 0.154 |
Total number of observations | 86388 | 2825 | 11901 |
Number of reflections | 28628 | ||
<I/σ(I)> | 18.9 | 44.3 | 4.6 |
Completeness [%] | 95.7 | 95.1 | 91 |
Redundancy | 3 | 2.9 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1.6M AMMONIUM SULFATE, 10% DIOXANE, 0.1M MES PH 6.0, DEOXY-BIG CHAP |