4S10
Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2011 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 37.598, 46.334, 76.438 |
Unit cell angles | 103.00, 92.03, 89.85 |
Refinement procedure
Resolution | 19.722 - 2.614 |
R-factor | 0.2275 |
Rwork | 0.223 |
R-free | 0.31250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.402 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.720 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmerge | 0.074 | 13.600 |
Number of reflections | 14057 | |
<I/σ(I)> | 8.8 | 5.7 |
Completeness [%] | 93.1 | 91.5 |
Redundancy | 1.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 288 | 30% PEG 1500, VAPOR DIFFUSION, temperature 288K |