4RWO

Crystal structure of the porcine OAS1 L149R mutant in complex with dsRNA and ApCpp in the AMP donor position

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.06450
Spacegroup name	P 43 21 2
Unit cell lengths	73.100, 73.100, 208.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.306 - 2.200
R-factor	0.204
Rwork	0.202
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.306
High resolution limit [Å]	2.200
Number of reflections	29636
Completeness [%]	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	100 mM Tris-HCl pH 8.0, 25% PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 291K