4REB
Structural Insights into 5' Flap DNA Unwinding and Incision by the Human FAN1 Dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 |
| Unit cell lengths | 104.770, 104.770, 127.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 90.700 - 4.200 |
| R-factor | 0.2321 |
| Rwork | 0.230 |
| R-free | 0.26210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.511 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.700 | 4.310 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.076 | |
| Number of reflections | 10793 | |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 99.3 | 99.88 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | micro-batch under oil | 300 | 0.1mM spermidine, 1% 1,6-Hexanediol, 22-24% PEG 3350, 0.1M BisTris Propane, micro-batch under oil, temperature 300K |
