4QIL
Crystal structure of the ROQ domain of human Roquin in complex with the Hmg19 stem-loop RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.100 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.600, 170.000, 51.100 |
Unit cell angles | 90.00, 97.00, 90.00 |
Refinement procedure
Resolution | 37.696 - 2.900 |
R-factor | 0.1976 |
Rwork | 0.195 |
R-free | 0.25090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qik |
RMSD bond length | 0.010 |
RMSD bond angle | 1.374 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | COMO |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.094 | 0.415 |
Number of reflections | 17984 | |
<I/σ(I)> | 9.1 | 1.7 |
Completeness [%] | 96.8 | 88.4 |
Redundancy | 2.1 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 0.1 M Tris-HCl, pH 8.2, 0.3 M calcium acetate, 12%-14% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |