4Q9U

Crystal structure of the Rab5, Rabex-5delta and Rabaptin-5C21 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9785
Spacegroup name	P 41 21 2
Unit cell lengths	174.821, 174.821, 148.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.781 - 4.618
R-factor	0.2561
Rwork	0.251
R-free	0.34270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ot3
RMSD bond length	0.015
RMSD bond angle	1.873
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.760
High resolution limit [Å]	4.600	9.890	4.600
Rmerge	0.117	0.048	0.943
Number of reflections	12699
<I/σ(I)>	7.2
Completeness [%]	97.6	98.3	95.8
Redundancy	9.8	10.6	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	5	289	1.0M NaH2PO4/K2HPO4, pH 5.0, hanging drop, temperature 289K