4OVN
Voltage-gated Sodium Channel 1.5 (Nav1.5) C-terminal domain in complex with Calmodulin poised for activation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.128, 99.006, 109.281 |
| Unit cell angles | 90.00, 106.11, 90.00 |
Refinement procedure
| Resolution | 28.700 - 2.800 |
| R-factor | 0.215 |
| Rwork | 0.211 |
| R-free | 0.28200 |
| Structure solution method | MAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 2.850 |
| High resolution limit [Å] | 2.798 | 2.800 |
| Rmerge | 0.116 | 0.745 |
| Total number of observations | 311500 | |
| Number of reflections | 48516 | |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 90.3 | 58.3 |
| Redundancy | 6.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 15% PEG 4K, 0.2 M MgSO4 and 10% glycerol |
