4OCT
Crystal structure of human ALKBH5 crystallized in the presence of Mn^{2+} and 2-oxoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-23 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.930, 57.234, 78.407 |
| Unit cell angles | 90.00, 101.07, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.280 |
| R-factor | 0.2405 |
| Rwork | 0.238 |
| R-free | 0.28410 |
| Structure solution method | direct placement |
| Starting model (for MR) | related to pdb entry 4O61 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.401 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.14) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.470 | 38.470 | 2.360 |
| High resolution limit [Å] | 2.280 | 8.830 | 2.280 |
| Rmerge | 0.127 | 0.011 | 0.011 |
| Total number of observations | 2299 | 13193 | |
| Number of reflections | 18808 | ||
| <I/σ(I)> | 13.3 | 52.5 | 2.1 |
| Completeness [%] | 99.9 | 98.9 | 100 |
| Redundancy | 7.3 | 6.6 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.005 M of each manganous sulfate and 2-oxyglutarate were added to the protein stock solution. Reservoir: 20% PEG-3350, 0.2 M diammonium phosphate, vapor diffusion, temperature 291K |
