4NJ4
Crystal Structure of Human ALKBH5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-29 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97889 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 67.100, 82.660, 89.171 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.990 - 2.020 |
Rwork | 0.165 |
R-free | 0.21810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Ensemble of PDB entries 4IE5 3s57 2iuw and 3THT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.5) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.090 |
High resolution limit [Å] | 2.020 | 4.350 | 2.020 |
Rmerge | 0.142 | 0.090 | |
Number of reflections | 33274 | ||
<I/σ(I)> | 5.9 | 16.5 | 2 |
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 6 | 6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 15% PEG 3350, 0.125 M potassium nitrate, 0.5 mM manganese (II) chloride, 2 mM UN9, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |